The study for selecting the appropriate environment to create the entire plant varieties purl Lan was conducted in the laboratory at the University of Agriculture and Forestry. The results showed that the sterilization of Lan purl bud by 0.10 percent HgCl2 in 10 minutes has made a clean sample rate with high (80 percent). Using the experimental Environment MS + Inositol 100mg/l + sugar 30g/l + GA3 0.3mg/l BA + 0.5 mg/l + agar 5,2g1, pH = 5.6 to 5.8 created for high regeneration sample rate (93.33 percent). Fast regeneration environment MS + Inositol 100 mg/l + sugar 30g/l + BA 1 mg/l + NAA 0.5mg/l + agar 5,2mg/l, pH = 5.6 to 5.8 created fast multiplier reached 5.9 times. Applying the rooting environment MS + Inositol 100mg/1 + sugar 30g / l + NAA 1 mg / l + agar 5,2g / l, pH = 5.6 to 5.8 created highest purl rate (3.6 roots). However, using MS + Inositol 100mg/l + sugar 30g / l + NAA 0.5 mg / l + agar 5,2g / l, pH = 5.6 to 5.8 created good quality of roots with fat and long.