Study on spiders in Dai Lai resort was undertaken in 2015. The sites have been chosen include the garden, plantation, lakeside and Ngoc island. A total of 419 individuals that belong to 6 families and 27 species were collected, among them, 24 species (135 adults) from garden, 17 species (63 adults) from plantation, 6 species (178 adults) from lakeside, and 9 species (43 adults) from Ngoc island. The results showed that the assemblage of spider was significantly different between sites.