Nghiên ứu áp dụng phản ứng fenton để phân tích hoạt tính chống oxy hóa

• Tác giả: Nguyễn Duy Bảo Huỳnh, Thị Hoài Dương Ngô, Thị Hiền Phạm
• Ngôn ngữ: vie
• Ký hiệu phân loại: 616.399 Other nutritional and metabolic diseases
• Thông tin xuất bản: Khoa học Công nghệ Thủy sản, 2014
• Mô tả vật lý: 45550
• Bộ sưu tập: Metadata
• ID: 655044

Tóm tắt

This study was to develop new method for determining antioxidant activity based on Fenton reaction. The reaction system included 50 uM eicosapentaenoic (EPA), 50 uM met-myoglobin (metMb) and 100 uM HP2. The maximum ferryl-myoglobin (ferryIMb) radical level was generated from the reaction between metMb and H2O2 after 180 sec. The incubation time for oxidation of EPA initiated by ferrylMb radical at 37oC was 30 min. Antioxidant activity of ESH determined by the Fenton reaction system was in correlation with those of DPPH radical scavenging activity (R2 = 0.99), total reducing power (R2 = 0.98) and H2O2 scavenging activity (R2 = 0,99).