Study on probiotic bacteria is a new trend in recent years. The probiotic bacteria has many advanced properties, especially some characteristics on colonization, producing bactericidal substance and growth inhibition on pathogenic bacteria. Probiotic bacteria isolated will be initial material for production of pro biotic products. This article showed the result of isolation probiotic bacteria strain from tiger shrimp gut (Penaeus monodon) and probiotic properties of isolated bacteria strain. Result from 16S rDNA identification revealed that the isolated bacteria were Pseudomonas stutzeri. This strain could produce bactericidal substance, colonize and inhibit the growth of pathogen bacteria V. alginolyticus and V. parahaemolyticus.