Trace elements in environmental matrices and their potential harm to humans and the ecosystem are causing growing concern. For environmental monitoring and assessment, trace element analysis in water, soil, and air must be precise. Due to its selectivity and sensitivity, fluorometric analysis has grown in popularity. This review covers the latest fluorometric approaches for trace element measurement in environmental samples. This review discusses sensitive and selective fluorometric probes. A significant increase in fluorescence intensity makes this probe better for trace element research. Various parameters like pH, temperature, and reaction time were carefully optimized in each approach to increase sensitivity and accuracy. The current study sheds light on this analytical approach's concepts, applications, and difficulties. In general, fluorometric analysis is important for environmental studies. For researchers, it allows them to use fluorometric technology with other methods and focus on real-time analysis to solve environmental challenges.