Padel is a worldwide racket sport that is growing each year. The unilateral upper limb repetitive movements of this sport may lead to shoulder structural and functional adaptations, which may alter muscle mechanics, however evidence is limited. We aimed to investigate if there is morphological, mechanical and functional shoulder symmetry between upper limbs of professional padel players. Fourthteen (7♂ and 7♀) professional Brazilian padel players were evaluated at supraspinatus muscle thickness (MT), stiffness, shoulder rotation range of motion (ROM), shoulder internal and external rotation peak torques and their ratios (ER/IR) and fatigue index torque. Subsequently, were compared all variables between dominant and non-dominant sides (α = 0.05). Total work, IR and ER absolute and normalised torque were higher in the dominant limb (