BACKGROUND: Masters athletes (MA) are typically considered healthier than age-matched non-athletes. However, limited evidence exists on the prevalence of chronic conditions in MA. METHODS: Masters athletes competing at the 2017 Australian Masters Games ( RESULTS: Overall, 817 MA (53.7 ± 10.6 y, 61% female) completed the survey with 48% reporting ≥1 chronic condition. Cardiovascular conditions were less prevalent in MA CONCLUSIONS: Cardiovascular and other chronic conditions prevalence was lower in MA compared to age-matched non-athletes, highlighting the value of promoting sport involvement in aging individuals as well as for continuation of participation in younger age groups into MA level. Greater participation of younger and female groups in masters sport to improve mental health, and inclusion of people who are not employed should be supported.