Diagnostic Performance and Impact on Antimicrobial Treatment of a Multiplex Polymerase Chain Reaction in Critically Ill Patients With Pneumonia: A Multicenter Observational Study (The MORICUP-PCR Study: Morocco ICU Pneumonia-PCR study).

 0 Người đánh giá. Xếp hạng trung bình 0

Tác giả: Redouane Abouqal, Younes Aissaoui, Ayoub Belhadj, Adnane Berdai, Ayoub Bouchama, Brahim Boukatta, Tarek Dendane, Ali Derkaoui, Mouhssine Doumiri, Ahmed Rhassane El Adib, Karim ElAidaoui, Meryem Essafti, Abdelhamid Hachimi, Mehdi Khaddouri, Mohammed Khallouki, Jamal Eddine Kohen, Naoufel Madani, Oumaima Mroune, Latifa Oualili, Mehdi Oudrhiri Safiani, Nabila Soraa, Amra Ziadi

Ngôn ngữ: eng

Ký hiệu phân loại: 912.01 Philosophy and theory

Thông tin xuất bản: United States : Critical care explorations , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 66741

 OBJECTIVES: Managing severe pneumonia remains a challenge. Rapid diagnostic tests, such as multiplex polymerase chain reaction (mPCR), facilitate quick microorganism identification and may enable timely and appropriate antimicrobial therapy. However, studies from low-income countries are scarce. This study aimed to evaluate the diagnostic characteristics of mPCR and its impact on antibiotic therapy and outcomes in critically ill patients with pneumonia. DESIGN: Multicenter observational study. SETTING: Twelve ICUs across Morocco. PATIENTS: Adult patients with pneumonia requiring invasive mechanical ventilation, including community-acquired pneumonia (CAP), hospital-acquired pneumonia (HAP), and ventilator-associated pneumonia (VAP). INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Respiratory samples were analyzed using both mPCR and conventional microbiological methods. The diagnostic performance of mPCR was evaluated, including its sensitivity and specificity. Additionally, the appropriateness of mPCR-induced modifications in empiric antibiotic therapy and their impact on patient outcomes were assessed. A total of 210 patients were included, with a median age of 50 years (range, 33-67 yr), of whom 66.2% were male. Pneumonia types were distributed as 30% CAP, 58% VAP, and 12% HAP. mPCR demonstrated a sensitivity of 96.9% (95% CI, 92.3-99.2%) and a specificity of 92% (95% CI, 91-93%). Following mPCR, antibiotic therapy modifications were observed in 58% of patients (n = 122), including de-escalation or cessation in 11% (n = 23), escalation in 26.5% (n = 56), adequacy adjustments in 7.5% (n = 16), and initiation of antibiotics in 13% (n = 27). The appropriateness of antibiotic therapy increased significantly from 38.7% (n = 83) to 67% (n = 141
  difference, 27.5%
  95% CI, 18.3-36.7
  p <
  0.0001). Generalized mixed model analysis revealed that appropriate post-mPCR antibiotic therapy was associated with reduced mortality (adjusted odds ratio, 0.37
  95% CI, 0.15-0.93
  p = 0.038). CONCLUSIONS: Our findings suggest that the use of mPCR is associated with a significant improvement in the appropriateness of empiric antibiotic therapy and is also associated with a positive impact on the outcome of patients with pneumonia.
Tạo bộ sưu tập với mã QR

THƯ VIỆN - TRƯỜNG ĐẠI HỌC CÔNG NGHỆ TP.HCM

ĐT: (028) 36225755 | Email: tt.thuvien@hutech.edu.vn

Copyright @2024 THƯ VIỆN HUTECH