BACKGROUND: Acute myocardial infarction (AMI) is a leading cause of mortality worldwide. Adipose-derived stem cell (ADSC) transplantation presents a promising therapeutic approach for AMI
however, the harsh microenvironment of the infarcted myocardium, characterized by hypoxia and oxidative stress, limits the survival and efficacy of ADSCs. Nanozymes (NZs), which have robust anti-oxidative enzyme-mimicking activities, have demonstrated potential in combating oxidative stress and improving cell viability. METHODS: Mn3O4 NZs (Mn-Nzs), which have nanoflower-like structures were synthesized and their structure and multi-enzyme mimetic activities (superoxide dismutase, catalase, and glutathione peroxidase) were characterized. Blood biochemical parameters were measured in the heart, liver, spleen, lungs and kidneys of the rats, followed by hematoxylin and eosin (HE) staining. The impact of Mn3O4 NZs on reactive oxygen species (ROS) levels, and viability of ADSCs under oxidative stress was assessed in vitro. In vivo studies were conducted using a rat AMI model to evaluate the therapeutic efficacy of ADSC transplantation, in conjunction with Mn3O4 treatment. In addition, proteomic analysis was performed to elucidate the mechanisms of action underlying the therapeutic effects. RESULTS: Mn CONCLUSION: The findings highlight a novel strategy integrating NZ anti-oxidant properties with stem cell transplantation to improve AMI treatment outcomes.