Ảnh hưởng của việc bổ sung vegf đến sự thành thục nhân của tế bào trứng heo

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Tác giả: Ngọc Tấn Nguyễn, Thị Ngọc Hân Nguyễn

Ngôn ngữ: vie

Ký hiệu phân loại: 571.845 +Gametogenesis

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật chăn nuôi, 2021

Mô tả vật lý: 45328

Bộ sưu tập: Metadata

ID: 673580

Thêm vào giỏ

In the present studythe effects of Vascular Endothelial Growth Factor (VEGF) protein supplementation in culture medium on porcine oocytes nuclear maturation in vitro was investigated. Cumulus oocyte complexes (COCs) were collected and cultured in TCM-199 medium containing 10% follicle fluid (FF)0.8% BSA and 10UI (hCGml) and supplemented with different concentrations of VEGF (050100200 ngml). The results showed that treated group COCs with VEGF protein as 200 ngml resulted in enhanced nuclear maturation rate (82.9%) as compared to control group (58.6%) or other treated groups with 50 ngml (65.7%) and 100 ngml (74.3%). Furthermorewe also found the stage-dependent effect for VEGF supplemented in culture medium on nuclear maturation. The maturation rate was highest (P<
0.01) in the treated group during 44h of culture (85.8%) as compared to none treated group (59.2%)treated group just for first 22h (75.0%) or the last 22h (66.7%). Taken togetherwe conclude that supplemented as 200 ngml of VEGF in the culture medium increases the nuclear maturation rate of porcine oocytes. Howeverin order to understand more insight into the cytoplasmic maturation requires more studies.Nghiên cứu nhằm đánh giá ảnh hưởng của yếu tố tăng trưởng nội mao mạch (VEGF: Vascular Endothelial Growth Factor) đến sự thành thục nhân tế bào trứng heo. Phức hợp tế bào trứng (cumulus oocyte complexes–COCs) có từ 2 lớp tế bào cumulus trở lên và đồng đều tế bào chất sẽ được nuôi cấy thành thục trong 44 giờ. Môi trường nuôi cấy là TCM-199 có bổ sung 10% dịch nang noãn + 0,8% BSA và 10 UI hCGml. Protein VEGF được bổ sung ở các nồng độ khác nhau (50100200 ngml) và không bổ sung VEGF (0 ngml) được xem là đối chứng. Kết quả cho thấy tỷ lệ thành thục nhân của tế bào trứng heo tăng dần khi tăng nồng độ VEGF 0-200 ngml. Tỷ lệ thành thục nhân cao nhất ở nhóm COC nuôi cấy có bổ sung 200 ngml và thấp nhất ở nhóm đối chứng (82,9 so với 58,6%P<
0,01) hoặc 65,7 hay 74,3% lần lượt ở nhóm COC bổ sung 50 và 100 ngml. Kết quả cũng cho thấy bổ sung protein VEGF (200 ngml) theo các giai đoạn nuôi cấy có ảnh hưởng đến tỷ lệ thành thục nhân. Tỷ lệ thành thục nhân cao nhất ở nhóm bổ sung VEGF suốt 44 giờ nuôi cấy (85,8%)tiếp đến là nhóm bổ sung ở 22 giờ đầu (75,0%)sau đó là nhóm bổ sung ở 22 giờ cuối (66,7%) và thấp nhất ở nhóm đối chứng (59,2%). Việc bổ sung VEGF giúp cải thiện tỷ lệ thành thục nhân tế bào trứng heo trong điều kiện in vitrocần tiếp tục nghiên cứu để làm rõ hơn vai trò của VEGF đến thành thục tế bào chất và phát triển phôi sau đó.

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