Viticulture, the science of growing, cultivating, and harvesting grapes, and enology, the art and science of making wine, are rapidly evolving through innovative approaches aimed at improving the quality and efficiency of grape and wine production. This review explores the emerging use of nanoparticles, in particular gold, silver, and magnetic nanoparticles, to improve the quality, safety, and sustainability of both grape growing and winemaking processes. The unique properties of these nanoparticles, such as their small size, high surface area, and distinct chemical properties, enable them to address key challenges within the industry. In viticulture, nanoparticles have shown potential in protecting vines from pathogens, optimizing grape yield, and improving quality. In enology, nanoparticles are making a significant contribution to microbial control, reducing spoilage and refining wine analysis techniques, leading to improved product quality and safety. This review also highlights the synergy between different types of nanoparticles and their diverse applications, from microbial control in wine production to their use in innovative packaging solutions. In addition, nanoparticles have the potential to reduce dependence on agrochemicals and improve the sustainability of wine production, which is a promising avenue for future research. However, the integration of nanoparticles in viticulture and enology also poses regulatory and safety challenges, including the potential for nanoparticles to leach into wine products. Further research and regulatory advances are essential to ensure the safe and effective use of these technologies in winemaking. Overall, nanoparticles offer significant benefits to the wine industry, driving improvements in efficiency, sustainability, and quality.