BACKGROUND: Ankle sprains during stair descent are prevalent, especially in those with chronic ankle instability (CAI), which may be attributed to diminished ankle proprioception associated with CAI. OBJECTIVE: This study aimed to determine whether individuals with CAI have lower ankle proprioceptive performance during stair descent and to determine to what extent stair riser height may affect ankle proprioception. METHODS: 40 university students, including 21 CAI (9 males and 12 males, mean age 22.38 years, mean height 169.97 cm and mean weight 64.88 kg) and 19 healthy controls (11 males and 8 males, mean age 23.05 years, mean height 169.42 cm and mean weight 65.18 kg) volunteered. The Ankle Inversion Discrimination Apparatus for Stair Descent (AIDASD) was used to measure ankle inversion proprioception across 3 different riser heights: 15 cm, 17.5 cm, and 20 cm during stair descent. RESULTS: ANOVA showed that individuals with CAI performed significantly worse than health group across all tested riser heights (F = 44.066, p <
0.001), with a significant main effect of riser height (F = 13.288, p <
0.001). Significant differences in proprioceptive acuity were found between 15 cm and higher risers ( CONCLUSION: The presence of ankle instability and increased riser height significantly negatively affected ankle inversion proprioceptive performance during stair descent, which may increase the risk of ankle sprain. APPLICATION: Potential applications of this research include the assessment of ankle proprioception during stair descent attributable to effective ankle instability rehabilitation and riser height selection for safe stair design.