Contribution of cuproptosis and immune-related genes to idiopathic pulmonary fibrosis disease.

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Tác giả: Qiong Feng, Chengji Jin, Jia Li, Qiaoyu Li, Yongjie Li, Jin Liang, Qiuli Nie, Jing Wang, Lipeng Zhang, Shaomao Zheng, Yu Zheng

Ngôn ngữ: eng

Ký hiệu phân loại: 262.924 Quinque compilationes antiquae

Thông tin xuất bản: Switzerland : Frontiers in immunology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 675222

BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a degenerative respiratory condition characterized by significant mortality rates and a scarcity of available treatment alternatives. Cuproptosis, a novel form of copper-induced cell death, has garnered attention for its potential implications. The study aimed to explore the diagnostic value of cuproptosis-related hub genes in patients with IPF. Additionally, multiple bioinformatics analyses were employed to identify immune-related biomarkers associated with the diagnosis of IPF, offering valuable insights for future treatment strategies. METHODS: Four microarray datasets were selected from the Gene Expression Omnibus (GEO) collection for screening. Differentially expressed genes (DEGs) associated with IPF were analyzed. Additionally, weighted gene coexpression network analysis (WGCNA) was employed to identify the DEGs most associated with IPF. Ultimately, we analyzed five cuproptosis-related hub genes and assessed their diagnostic value for IPF in both the training and validation sets. Additionally, four immune-related hub genes were screened using a protein-protein interaction (PPI) network and evaluated through the receiver operating characteristic (ROC) curve. Lastly, single-cell RNA-seq was employed to further investigate differential gene distribution. RESULTS: We identified a total of 92 DEGs. Bioinformatics analysis highlighted five cuproptosis-related genes as candidate biomarkers, including three upregulated genes ( CONCLUSION: The research methodically depicted the intricate interplay among five cuproptosis-related genes, four immune-related hub genes, and IPF, offering new ideas for diagnosing and treating patients with IPF.
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