INTRODUCTION: Type 2 diabetes mellitus (T2DM) is a substantial worldwide health issue characterized by chronic hyperglycemia and secondary health complications. The current investigation intends to assess the in vivo hypoglycemic potential of selected medicinal herbs ( METHODOLOGY: Male Wistar albino rats have been allocated into standard and experimental groups, receiving individual herb crude powder and PHF at different doses, i.e., 25, 50, 75, and 100 mg/kg of dose on post-oral sucrose-loaded (5 mg/kg of body weight) rats. The blood glucose levels have been assessed every 0, 30, 60, 90, and 120 minutes. The area under the curve (AUC) was determined. Both sensory and proximate evaluations were performed on the bread. RESULT: The results demonstrated a significant (p<
0.05) lowering in the blood glucose levels with 75 and 100 mg/kg of dose at different time intervals in rats treated with PHF in contrast to the control group, while the administration of individual herbs does not illustrate any noteworthy changes in the levels of blood glucose except CONCLUSION: The combination of herbs showed a synergistic effect, leading to enhanced glucose regulation in an oral sucrose-loaded rat model, and PHF at 75 and 100 mg/kg of dose possess better hypoglycemic activity as compared to individual herbs. The utilization of PHF in dietary products may be recommended as a nutraceutical product for the mitigation of T2DM.