Host species, locations, and diet can significantly impact microbial diversity and community in insects. Several ladybird beetles are known as key predators and potential biological control agents for aphids. However, there is limited understanding of how host species, locations, and aphid prey influence the microbial diversity and community of aphidophagous ladybird beetles in natural environments. In this study, we collected 74 samples of ladybirds and their aphid prey from various locations in Guangxi, China, and sequenced the 16S amplicons to investigate differences in their microbiomes. The dominant genera in the ladybird samples,