BACKGROUND: Metabolic dysfunction-Associated Steatotic Liver Disease (MASLD) affects up to one in five people in the UK, with persistent overeating and a sedentary lifestyle being significant risk factors. Exploring dietary patterns at a food level is a novel approach to understand associations between diet and disease. METHODS: This cross-sectional case-control study included 168 MASLD patients and 34 healthy controls from Nottingham (UK). Dietary data were collected using the EPIC-food frequency questionnaire. A food-group, tree classification method was developed which categorized 923 ingredients into three levels (main food group, sub-types, and cooking methods) and intakes were associated with clinical outcomes using logistic regression and degree of liver fibrosis using linear regression. RESULTS: Significant associations were found for red meat intake with MASLD (OR [CI]: 1.013 [1.001-1.025]) and fibrosis (Beta [SE]: +0.048 [0.013])
intakes of nuts (OR [CI]: 0.951 [0.905-0.999])
and fish (OR [CI]: 0.985 [0.971-0.999]) with MASLD
"Cereals and cereals products", "salt and gravy" and baked foods with fibrosis (Beta [SE]: +0.018 to +0.057 [0.005-0.23])
white and organ meat (Beta [SE]: -0.04 to -0.61 [0.015-0.249])
diet soda (OR [CI]: +0.01 [1-1.003]) and red meat intakes (OR [CI]:+0.002 [1.002-1.016]) with T2DM
wholegrain wheat, red meat, and semi-skimmed dairy intakes with hypercholesterolemia (ORs [CI]: -0.003 to -0.023 [1-1.043])
"herbs and spices" and wholegrain rice with hypercholesterolaemia (ORs [CI]: -0.08 to -0.98 [0.159-0.989)
fresh herbs and boiled foods intakes with hypertension (ORs [CI]: -0.001 to -2.21 [0.013-1]). CONCLUSION: The study introduces a new food-group, tree classification method to characterise UK diet data and identify risk factors for MASLD, potentially informing the development of culturally applicable dietary guidelines designed to improve public health.