Differences in diversity and composition of mucosa-associated colonic microbiota in colorectal cancer and non-colorectal cancer in Indonesia.

• Tác giả: Murdani Abdullah, Nikko Darnindro, Achmad Fauzi, Dadang Makmun, Erni J Nelwan, Saskia A Nursyirwan, Amanda Pitarini, Ikhwan Rinaldi, Cleopas M Rumende, Hamzah Shatri, Ninik Sukartini, Caroline Tanadi
• Ngôn ngữ: eng
• Ký hiệu phân loại: 729.6 Decoration in veneer and incrustation
• Thông tin xuất bản: United States : World journal of gastroenterology , 2025
• Bộ sưu tập: NCBI
• ID: 675842

Tóm tắt

BACKGROUND: Colorectal cancer is the third most common malignancy and the fourth leading cause of cancer-related deaths worldwide. Several studies have shown an association between gut microbiota and colorectal cancer. Gut microbiota is unique and can be influenced by geographic factors and habits. This study aimed to determine the diversity and composition of colonic mucosal microbiota in patients with and without colorectal cancer. AIM: To determine the diversity and composition of colonic mucosal microbiota in patients with and without colorectal cancer in Indonesia. METHODS: This case-control study included 59 subjects (35 colorectal cancer patients and 24 non-colorectal cancer patients indicated for colonoscopy at Dr. Cipto Mangunkusumo Gastrointestinal Endoscopy Center and Fatmawati Hospital. Microbiota examination was performed using 16S rRNA sequencing. Bioinformatics analysis was performed using the wf-metagenomics pipeline from EPI2Me-Labs (Oxford Nanopore Technologies platform). RESULTS: Patients with colorectal cancer had a higher median index value on the Shannon index (3.28 CONCLUSION: Patients with colorectal cancer exhibit distinct differences in the composition and diversity of their colonic mucosal microbiota compared to those with non-colorectal cancer. This study was reviewed and approved by the Ethics Committee of Faculty of Medicine, Universitas Indonesia (No. KET-1517/UN2.F1/ETIK/PPM.00.02/2023).