INTRODUCTION: Amidst the rapid digitalization of healthcare, there is a need for tools that accurately assess eHealth literacy across cultural contexts. This study focused on the validation of an Arabic version of the eHealth Literacy Questionnaire (eHLQ), a tool to facilitate patient engagement and health outcomes in digital healthcare. METHOD: Using a convenience sampling method, the study recruited a diverse sample of 657 participants from Jordan (58.9% females and 41.1% males). RESULTS: Confirmatory Factor Analysis and Rasch analysis supported a six-factor model and demonstrated satisfactory item performance within established thresholds. The findings revealed good internal consistency with Cronbach's α ranging between ranging between 0.71 to 0.84. Differential Item Functioning analysis indicated no gender-specific variations. DISCUSSION: The validated Arabic eHLQ is a reliable tool that can help in supporting the development of tailored interventions to improve healthcare delivery in Arabic-speaking regions.