Infectious diseases have emerged as a significant global concern, posing a substantial burden in terms of high morbidity and mortality and presenting considerable challenges in clinical diagnosis and treatment. Therefore, it is highly desired to develop new strategies for sensitive and accurate bacteria detection to address the global epidemic of antibiotic resistance. In this study, a new technique utilizing a dual proximity ligation mediated chain extension and displacement strategy was developed for precise identification and highly sensitive detection of Methicillin-Resistant