BACKGROUND/OBJECTIVES: Adequate hydration is fundamental for maintaining physical and mental health, yet dehydration remains a common issue, with significant health consequences such as fatigue, impaired cognitive function, and increased risk of chronic diseases. This study aimed to assess the water balance status of healthy employees in Greece and explore the influence of sociodemographic, anthropometric, occupational, and other lifestyle factors. METHODS: After completing a validated questionnaire, demographic, dietary, and occupational factors were first analyzed by gender and then examined for their association with water balance, defined as the net difference between water intake and losses, using univariate and multivariable analyses. RESULTS: Daily water consumption had a median value of 3063.77 mL (Q1: 2181.62, Q3: 4070.04), with men consuming significantly more than women. Multivariable analysis identified several factors associated with water balance, including years of education (β: -21.88, SE: 8.55), hours of work per week (β: 30.17, SE: 15.38), use of glasses during the day (Yes vs. No: β: 423.13, SE: 216.41), use of a bottle during the day (Yes vs. No: β: 873.50, SE: 278.82), and consuming water for pleasure (Yes vs. No: β: 478.63, SE: 200.16). Additionally, exposure to weather conditions at work (Yes vs. No) was suggested to have a positive influence on water balance. CONCLUSIONS: This study underscores the complex interplay between sociodemographic and occupational factors and hydration practices, providing evidence for targeted hydration strategies, as wellness programs and public health initiatives aimed at improving hydration among working populations in Greece.