How a disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization.

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Tác giả: Ashish Bihani, Borries Demeler, Nicole J Francis, Tim M Gemeinhardt, Amy Henrickson, David Jeruzalmi, Utkarsh Kapoor, Chongwoo A Kim, Young C Kim, Heather J Ledterman, Daniel Lopes, Andrea J Mendiola, Jeetain Mittal, Nanu Pal, Tien M Phan, Roshan M Regy, Olga Senkovich, Jyoti Sharma, Djamouna Sihou

Ngôn ngữ: eng

Ký hiệu phân loại: 973.928 Administration of George Bush, 1989-1993

Thông tin xuất bản: United States : bioRxiv : the preprint server for biology , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 677095

Biomolecular condensates are increasingly appreciated for their function in organizing and regulating biochemical processes in cells, including chromatin function. Condensate formation and properties are encoded in protein sequence but the mechanisms linking sequence to macroscale properties are incompletely understood. Cross species comparisons can reveal mechanisms either because they identify conserved functions or because they point to important differences. Here we use in vitro reconstitution and molecular dynamics simulations to compare Drosophila and human sequences that regulate condensate formation driven by the sterile alpha motif (SAM) oligomerization domain in the Polyhomeotic (Ph) subunit of the chromatin regulatory complex PRC1. We discover evolutionarily diverged contacts between the conserved SAM and the disordered linker that connects it to the rest of Ph. Linker-SAM interactions increase oligomerization and regulate formation and properties of reconstituted condensates. Oligomerization affects condensate dynamics but, in most cases, has little effect on their formation. Linker-SAM interactions also affect condensate formation in Drosophila and human cells, and growth in Drosophila imaginal discs. Our data show how evolutionary sequence changes in linkers connecting conserved structured domains can alter condensate properties.
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