Digital enzyme-linked immunosorbent assays (dELISAs) very sensitively detect biomarkers that cannot be measured using traditional methods. The molecules are confined within a small volume, their counts accurately computed, and the results rapidly delivered. Digital ELISAs find many applications. In recent years, such ELISAs have become increasingly used to aid ophthalmological diagnoses and treatments, and have revolutionized the field. This article reviews the applications of dELISAs in clinical practice, especially in the sphere of ophthalmology.