PURPOSE: Given the high prevalence of food insecurity among college students, there is an interest in identifying whether the use of a single item can adequately screen for food insecurity. The current study aimed to determine the validity of a single-item food insecurity screening question among college students. DESIGN: Cross-sectional study exploring food insecurity among racial and ethnic undergraduate college students. SETTING: Large urban U.S. research university. SAMPLE: Undergraduate students (n = 667) 18 years or older. MEASURES: Food Insecurity screening was assessed via a single-item from the USDA Household Food Security Short Form (USDA FSSM-SF). Food security was assessed via the USDA FSSM-SF, a validated six-item scale that assesses food insecurity and hunger. ANALYSIS: Logistic regression assessed the validity of the single-item food insecurity question tested against the USDA FSSM-SF. RESULTS: The full model was statistically significant, χ2(1) = 161.44, CONCLUSION: Results support for the test validity of a single-item screening question that can be used to detect food insecurity among college students and inform secondary prevention programs aimed at food insecurity.