Design of immunoassay based biosensor platforms for SARS-CoV-2 detection using highly specific monoclonal antibodies.

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Tác giả: Esin Akçael, Göknur Gizem Dinç, İlkay Göksu Polat, Ebru Saatçi, Uygar Halis Tazebay, Fatıma Yücel

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: United States : Diagnostic microbiology and infectious disease , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 678084

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The global expand of SARS-CoV-2 has highlighted the importance of early and rapid detection to control the spread of a pandemic. In this study, specific and high-affinity monoclonal antibodies (mAbs) were developed against the conserved nucleocapsid protein of the virus among variants. Appropriate antibody pairs were selected to develop a lateral flow immunoassay (LFIA) and an unconventional application of an amperometric biosensor using unmodified screen-printed electrodes and external magnetic bead preparation. In the study, the LFIA we developed detected the SARS-CoV-2 virus at 10

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