Immunodominant Molecules for the Immunodiagnosis of Strongyloides stercoralis Infection.

• Tác giả: Maria Gabriela Almeida, Weslei Almeida Costa Araújo, Nilo Manoel Pereira Vieira Barreto, Joelma Nascimento de Souza, Victor Amazonas Pereira Maraux, Cíntia de Lima Oliveira, Carina da Silva Pinheiro, Neci Matos Soares, Joana R Sousa, Márcia Cristina Aquino Teixeira
• Ngôn ngữ: eng
• Ký hiệu phân loại: 333.9111—.9117 Other natural resources
• Thông tin xuất bản: United States : Diagnostic microbiology and infectious disease , 2025
• Bộ sưu tập: NCBI
• ID: 678123

Tóm tắt

 This study aimed to identify immunodominant molecules with potential for S. stercoralis infection immunodiagnosis. Serum samples were tested by an IgG-ELISA using Strongyloides venezuelensis soluble (AgS) and membrane (AgM) antigens, and its protein fractions (<
 100 and >
 100 kDa). Proteins were tested by one- and two-dimensional (2D) gel immunoblotting. The ELISA results showed sensitivities, ranging from 82.3 to 85.3 %, while specificities ranged from 60.0 to 93.3 %. Cross-reactivities were observed with sera from individuals infected with other helminths. Immunoreactive protein bands were revealed by Western blotting using both antigens, and a band with molecular weight around 140 kDa of AgS showed 100 % sensitivity and specificity, without cross-reactivity. The 2D immunoblotting revealed 7 spots in the 140 kDa protein with the S. stercoralis pool of serums. The pre-selection of Strongyloides immunodominant proteins that are highly specific and free from cross-reactions is essential for the production of recombinant antigens for the immunodiagnosis of strongyloidiasis.