OBJECTIVE: This study aimed to determine the genotypic characteristics of patients with hereditary spherocytosis (HS) in Türkiye and to examine the correlation between genotype and phenotype. MATERIALS AND METHODS: We analyzed the cases of 18 patients admitted to the pediatric hematology outpatient clinic with hemolytic anemia, jaundice, cholelithiasis, and splenomegaly. According to the Eber classification, the patients' clinical presentations were categorized as mild, moderate, or severe. Next-generation sequencing was used to analyze single-nucleotide and copy-number variations in all genes associated with HS via clinical exome sequencing. Relationships between the genes with detected variants and the clinical presentations of the patients were investigated. RESULTS: In total, 21 variants were detected in 5 HS-related genes. Twelve of them were previously reported variants and 9 were novel variants. Seven of them were pathogenic and two were classified as variants of uncertain significance according to the American College of Medical Genetics and Genomics. We discuss the phenotypic effects of novel pathogenic variants in the CONCLUSION: Molecular diagnosis of HS is important for treatment, prediction of the clinical outcome, and appropriate genetic counseling. Our study contributes to knowledge of the genotype-phenotype distribution of HS by introducing novel variants to the literature.