Toxoplasmosis, caused by the intracellular parasite Toxoplasma gondii, is a globally prevalent infection. The clinical manifestations are often asymptomatic, particularly in immunocompetent patients. Human testicular toxoplasmosis is an extremely rare presentation occasionally observed in immunocompromised patients. This case report presents a 32-year-old immunocompetent male with a history of right radical orchiectomy for a mixed germ cell tumor who presented with acute left testicular pain and was diagnosed with testicular toxoplasmosis.