Although bench-scale studies have shown that superheated steam is effective for microbial inactivation on surfaces, commercial systems in the hands of human operators have not been evaluated. The first aim of this study was to characterize the temperature of stainless-steel surfaces treated with a commercial unit. The geometric center of the stainless-steel surface was treated with superheated steam at 400 °C from a fixed position. Surface temperatures exceeded 300 °C at the impingement point during 5 min exposure but decreased as surface thickness and distance increased. Ambient temperature (23.5, 12.8, 4 °C) negatively impacted surface temperatures. Next, we evaluated the thermal inactivation of spot-inoculated Enterococcus faecium NRRL B-2354 on stainless steel surfaces. Inactivation of E. faecium decreased from 9.6 ± 0.1 log CFU/cm