Exposure to organophosphate esters (OPEs) remains a significant concern, especially in aquatic systems where these compounds can bioaccumulate in organisms. However, limited information exists regarding their potential health risks to humans through the food web in tropical ecosystems. This study investigated the levels, bioaccumulation, and trophic transfer of OPEs in sediments and marine fish species from Cartagena and Barbacoas bays, Colombia. Twenty target OPEs were quantified in sediments and fish. The average concentrations of ∑