Nitrogen (N) is the driving factor for crop yield and quality, and more research is needed on the mechanisms of aged micro/nano plastics (MNPs) on N assimilation in edible crops. In this study, pot experiments were conducted to investigate the potential effect of aged polyethylene (PE) microplastic addition (particle sizes: 20 and 0.1 µm, addition levels: 0.5 % [w/w], referred to as the control (CK), P20 (20-µm PE), AP20 (20-µm aged PE), P0.1 (0.1-µm PE), AP0.1 (0.1-µm aged PE) on MNPs accumulation and N assimilation in romaine lettuce (Lactuca sativa L.). The results showed that the particle size of MNPs accumulated in lettuce decreased from root >
stem >
leaf. Compared to CK, the fresh plant weight significantly decreased by 40.84 and 51.62 % in AP20 and AP0.1, respectively. The results indicated that MNPs could affect lettuce growth via soil nutrient availability, and aged 100-nm PE decreased soil NH