OBJECTIVE: To capture veterinary professionals' perspectives and applications of AI in veterinary care. This study assesses the perceived benefits, challenges, and potential areas where AI could enhance veterinary medicine and practice workflows. METHODS: An online survey was distributed to members of the American Animal Hospital Association and Digitail's network of veterinary professionals. The questionnaire included 18 close-ended and 7 open-ended questions exploring awareness, perceptions, usage, expectations, and concerns about AI in veterinary medicine. The survey was open from December 19, 2023, through January 8, 2024. RESULTS: The survey gathered 3,968 responses from professionals in various veterinary roles. Most respondents were veterinarians and veterinary technicians, with an average age of 35. CONCLUSIONS: Respondents demonstrated varying familiarity with AI, with an overall positive outlook toward its adoption in veterinary medicine. Those who actively use AI tools in their professional tasks reported higher levels of optimism about its integration. Key concerns included the reliability and accuracy of AI in diagnosis and treatment. The top benefits identified by respondents included improving efficiencies, streamlining administrative tasks, and potential contributions to revenue growth, employee satisfaction, and client retention. CLINICAL RELEVANCE: The findings underscore the influence of practical exposure and experience with AI tools on attitudes toward AI adoption. The positive correlation suggests that familiarity with AI technologies fosters trust and confidence, consequently driving greater acceptance and adoption within the veterinary community.