Exosome is a kind of membranous vesicles released from cells and carry a number of important signaling molecules, they play an important role in cellular communication, cell migration, angiogenesis as well as tumor cell growth. Exosome-based cancer diagnosis is usually achieved by detecting exosomal nucleic acids, lipids, and surface proteins, as they reflect tumor type and progression. Here, we proposed a method to rapidly prepare an array of washable magnetic nanoparticles (magnetic beads, MBs) by a magnetic field controlled system, which facilitate the analyzing of exosome phenotypes via super-resolution tricolor fluorescence co-localization (SR-TFC) and pixel counting (CFPP). Firstly, nanopore arrays were designed and prepared by 3D printing technology. MBs@SiO