In recent years, the use of priming agents, such as silicon, melatonin, salicylic acid, glycine betaine, and ascorbic acid has gained significant attention for their role in mitigating abiotic stresses across various plant species. While previous research has been conducted on the individual impact of silicon, melatonin, salicylic acid, glycine betaine, and ascorbic acid in metal stress resistance among various crop species, their combined effects in the context of heavy metal stressed conditions remain underexplored. Wheat (Triticum aestivum L.) seedlings was grown under the toxic concentration of copper (Cu) i.e., 100 µM which were applied with silicon, melatonin, salicylic acid, glycine betaine, and ascorbic acid under hydroponic conditions for 21 days. The research outcomes indicated that the toxic concentration of Cu in the nutrient solution notably reduced plant growth and biomass, photosynthetic pigments, and gas exchange attributes. However, Cu stress also induced oxidative stress in the plants by increasing malondialdehyde (MDA), hydrogen peroxide (H