Comparison of sample pretreatments used to distinguish between infectious and non-infectious foodborne viruses by RT-qPCR.

• Tác giả: Émilie Douette, Valérie Goulet-Beaulieu, Fabienne Hamon, Julie Jean, Éric Jubinville, Antoine Labrie, Anne-Marie Lauzier
• Ngôn ngữ: eng
• Ký hiệu phân loại: 629.47 Astronautical engineering
• Thông tin xuất bản: Netherlands : Journal of virological methods , 2025
• Bộ sưu tập: NCBI
• ID: 680463

Tóm tắt

To detect viruses such as hepatitis A virus (HAV) and human norovirus (HuNoV) in foods, RT-qPCR or other molecular methods are used, which cannot distinguish between infectious and non-infectious virions. Samples can be pretreated to limit detection to intact and presumably infectious virions. We compared propidium monoazide (PMA or PMAxx), platinum (IV) chloride (PtCl