Using fertilizers and plant protection products in agriculture is a primary source of metal and metalloid contamination in agricultural landscapes. Monitoring of these areas is especially crucial for the production of healthy food. In this paper, we analyzed the occurrence of trace elements commonly considered as indicators of environmental pollution in the pellets of the apex predator of farmland, representing the group of birds of prey whose pellets have not been studied in this respect so far. We used 60 pellets of the Eurasian Kestrel (Falco tinnunculus) from 20 sites, to investigate the pollutants present in the extensive agricultural landscape of eastern Poland. Further, we developed a strategy for homogenizing and digestion of the samples and proposed the sample preparation protocol of them. We determined the optimal working conditions for using inductively coupled plasma mass spectrometry (ICP-MS) to measure the content of elements identified as environmental pollutants, such as As, Cd, Cr, Cu, Mn, Ni, Pb, Se, and Zn. The obtained level of the investigated elements indicates low pollution of the farmland in eastern Poland by Cd, Cr, Ni, Pb, Se, and Zn and slightly elevated concentrations of Cu and Mn resulting from using artificial fertilizers and plant protection products. This study has shown that bird pellet analysis is a practical, non-invasive tool for studying the pollution of the environment with chemical elements. Studying bird pellets in the context of farmland contamination provides insights into the level of environmental pollution and the potential impact of agricultural activities on ecosystems.