Structural Basis for the Catalysis and Substrate Specificity of a LarA Racemase with a Broad Substrate Spectrum.

• Tác giả: Benoit Desguin, Santhosh Gatreddi, Robert P Hausinger, Jian Hu, Julian Urdiain-Arraiza
• Ngôn ngữ: eng
• Ký hiệu phân loại: 624.171 Structural analysis
• Thông tin xuất bản: United States : ACS catalysis , 2025
• Bộ sưu tập: NCBI
• ID: 681473

Tóm tắt

The LarA family consists of diverse racemases/epimerases that interconvert the diastereomers of α-hydroxyacids by using a nickel-pincer nucleotide (NPN) cofactor. The hidden redox reaction catalyzed by the NPN cofactor makes LarA enzymes attractive engineering targets for various applications. However, how a LarA enzyme binds its natural substrate and recognizes different α-hydroxyacids has not been elucidated. Here, we report three high-resolution structures of the enzyme-substrate complexes of a broad-spectrum LarA enzyme from