BACKGROUND: The present study was conducted from 2021 to 2023 to analyze the clinical and laboratory characteristics of neurosyphilis and determine the risk factors associated with this condition. METHODS: A total of 400 patients were enrolled, including 100 individuals diagnosed with neurosyphilis, 100 without neurosyphilis, 100 with latent syphilis, and 100 healthy control subjects. Comparative analysis methods, serum VDRL titration, and correlation analysis were employed during the study. RESULTS: The analysis revealed significant differences in symptoms of central nervous system (CNS) involvement and serum VDRL titers among the patient groups. For instance, symptoms indicative of CNS impairment were more frequently observed in patients with neurosyphilis, and serum VDRL titers were statistically significantly higher in this same group. CONCLUSIONS: The obtained results can be utilized in clinical practice to enhance the accuracy of neurosyphilis diagnosis and management, thereby contributing to the improvement of early detection of this condition and prevention of its complications. The study allows for the inference of the importance of early detection of (CNS) dysfunction symptoms in patients (neurosyphilis) with syphilis and identifies key factors influencing the development of this disease. The findings hold significance for the diagnosis and treatment of neurosyphilis.