Genome-wide CRISPR guide RNA design and specificity analysis with GuideScan2.

• Tác giả: Vineet Bansal, Dimitar Chakarov, Christina S Leslie, Scott W Lowe, Haralambos Mourelatos, Yuri Pritykin, Francisco J Sánchez-Rivera, Henri Schmidt, Andrea Ventura, Minsi Zhang
• Ngôn ngữ: eng
• Ký hiệu phân loại: 629.231 Analysis and design
• Thông tin xuất bản: England : Genome biology , 2025
• Bộ sưu tập: NCBI
• ID: 681501

Tóm tắt

We present GuideScan2 for memory-efficient, parallelizable construction of high-specificity CRISPR guide RNA (gRNA) databases and user-friendly design and analysis of individual gRNAs and gRNA libraries for targeting coding and non-coding regions in custom genomes. GuideScan2 analysis identifies widespread confounding effects of low-specificity gRNAs in published CRISPR screens and enables construction of a gRNA library that reduces off-target effects in a gene essentiality screen. GuideScan2 also enables the design and experimental validation of allele-specific gRNAs in a hybrid mouse genome. GuideScan2 will facilitate CRISPR experiments across a wide range of applications.