The transition to renewable energy exacerbates direct land occupation by infrastructure, leading to habitat degradation and biodiversity loss. However, biodiversity loss driven by the production and consumption of different renewable energy deployment scenarios remains largely unquantified. Quantifying biodiversity loss associated with land occupation of renewable energy infrastructure is essential for a sustainable energy transition. Here, we developed a novel data set to evaluate renewable energy-related biodiversity loss by considering the current infrastructure setting and future development pathways. We found that the land occupation of renewable energy infrastructure resulted in global biodiversity loss equivalent amounting to 19 × 10