IL-1β drives SARS-CoV-2-induced disease independently of the inflammasome and pyroptosis signalling.

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Tác giả: Cody C Allison, James P Cooney, Kathryn C Davidson, Merle Dayton, Marcel Doerflinger, Smitha Rose Georgy, Marco J Herold, Stefanie M Bader, Liana Mackiewicz, Marc Pellegrini, Jan Schaefer, Lena Scherer, Andreas Strasser

Ngôn ngữ: eng

Ký hiệu phân loại:

Thông tin xuất bản: England : Cell death and differentiation , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 681870

Excessive inflammation and cytokine release are hallmarks of severe COVID-19. Certain programmed cell death processes can drive inflammation, however, their role in the pathogenesis of severe COVID-19 is unclear. Pyroptosis is a pro-inflammatory form of regulated cell death initiated by inflammasomes and executed by the pore-forming protein gasdermin D (GSDMD). Using an established mouse adapted SARS-CoV-2 virus and a panel of gene-targeted mice we found that deletion of the inflammasome (NLRP1/3 and the adaptor ASC) and pore forming proteins involved in pyroptosis (GSDMA/C/D/E) only marginally reduced IL-1β levels and did not impact disease outcome or viral loads. Furthermore, we found that SARS-CoV-2 infection did not trigger GSDMD activation in mouse lungs. Finally, we did not observe any difference between WT animals and mice with compound deficiencies in the pro-inflammatory initiator caspases (C1/11/12
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