This study investigated the probiotic properties and antibiofilm potential of Streptococcus thermophilus strains obtained from homemade and commercial dahi. The S. thermophilus strain from homemade dahi had greater acid tolerance than the commercial strain, indicating a greater capacity to live in the acidic environments of the stomach. The commercial strain had increased survivability in bile salts and was more hydrophobic than the homemade strain. These findings suggest improved adaptability and increased colonization in the gut. The genomes of both strains included genes associated with probiotic characteristics implying that the two strains may provide unique probiotic advantages. These findings highlight the importance of cell-free supernatants (CFS) of these strains in reducing biofilm formation of pathogenic bacteria. Gas chromatography-mass spectrometry demonstrated that 2, 4-di-tert-butylphenol was a shared metabolite in the CFSs of both strains
however, 2-butanol was found only in the CFS of the homemade dahi strain. In-silico investigations revealed that compounds have drug-like characteristics, suggesting that they could be used for treating biofilm-associated diseases. This study highlights the health advantages of probiotics found in traditional dahi, but it also provides a way to develop natural antibacterial medicines.