Oil sands process-affected water (OSPW) is a by-product of bitumen extraction from oil sands surface mining in Alberta, Canada. A major group of organics in OSPW known as naphthenic acid fraction compounds (NAFCs) are of concern due to their persistence and toxicity. Constructed wetland treatment systems have emerged as potential biological treatment approaches for reducing NAFC concentrations within OSPW. In this study, greenhouse-scale mesocosms simulating a constructed wetland consisting of coarse sand tailings (CST) and OSPW were used to evaluate the ability of Scirpus microcarpus, Triglochin maritima, and unplanted controls to attenuate NAFCs under spring/fall and summer temperatures (10°C/5°C and 20°C/10°C day/night). Overall, in this mesocosm system, NAFC attenuation was similar regardless of different design parameters such as plant type, plant presence, and temperature. By the end of the study, NAFCs attenuation was 30% to 50% lower than the initial OSPW depending on plant species, plant presence, and temperature. The relative abundance of the acutely toxic O