Corticosteroid and antimicrobial therapy in macrolide-resistant pneumococcal pneumonia porcine model.

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Tác giả: Marta Arrieta, Enric Barbeta, Gianluigi Li Bassi, Denise Battaglini, Joaquim Bobi, Roberto Cabrera, Pierluigi di Giannatale, Laia Fernández-Barat, Kasra Kiarostami, Blanca Llonch, Daniel Martinez, Ignacio Martín-Loeches, Andrea Meli, Ana Motos, Stefano Nogas, Montserrat Rigol, Nona Rovira-Ribalta, Giacomo Tanzella, Antoni Torres, Carmen Rosa Vargas, Jordi Vila, Hua Yang, Minlan Yang

Ngôn ngữ: eng

Ký hiệu phân loại: 618.9706 Pediatrics and geriatrics

Thông tin xuất bản: Germany : Intensive care medicine experimental , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 682004

BACKGROUND: Streptococcus pneumoniae, a primary cause of community-acquired pneumonia (CAP), is typically treated with β-lactams and macrolides or quinolones. Corticosteroids are now recommended as adjunctive therapy in severe CAP to improve outcomes. In this prospective randomized animal study, we evaluated the bactericidal efficacy of various antibiotic regimens combined with corticosteroids using a porcine pneumococcal pneumonia model. RESULTS: In 30 White-Landrace female pigs, pneumonia was induced by intrabronchial inoculation of macrolide-resistant S. pneumoniae 19A isolate. Animals were randomized to receive saline, ceftriaxone (CRO) with levofloxacin (LVX), CRO with azithromycin (AZM), or combinations of these with methylprednisolone (MP). The primary outcome, S. pneumoniae concentrations in lung tissue after 48 h of treatment, showed that the CRO + LVX, CRO + AZM, CRO + LVX + MP, and CRO + AZM + MP groups were equally effective in reducing bacterial load. However, complete bacterial eradication from lung tissue was achieved only in the CRO + AZM + MP group. Secondary outcomes, including bacterial burden in tracheal aspirates and bronchoalveolar lavage (BAL) samples, showed similar bactericidal activity across all treatment groups. The CRO + AZM + MP group demonstrated the most controlled inflammatory response, achieving baseline levels of inflammation, while other groups exhibited elevated inflammatory markers. CONCLUSIONS: Despite using a macrolide-resistant S. pneumoniae isolate, the combination of CRO, AZM, and MP achieves similar or even superior results compared to other antibiotic combinations. This regimen provides both bactericidal and immunomodulatory benefits, suggesting its effectiveness in treating macrolide-resistant S. pneumoniae pneumonia.
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