Microglia-mediated neuroinflammation is a key contributor to Parkinson's disease (PD) pathogenesis. Leucine-rich repeat kinase 2 (LRRK2), the leading genetic contributor to both familial and sporadic PD, has been implicated in driving this connection. However, its precise role remains incompletely understood due to technical challenges. To address this, we utilized a bacterial artificial chromosome (BAC) transgenic mouse model overexpressing human LRRK2-R1441G, which replicates key features of PD. These mice were crossed with Cx3cr1-EGFP mice to enable assessment of microglial dynamics and function using two-photon imaging in awake mice