The expansion of linear infrastructure presents a significant threat to biodiversity, emphasizing the urgent need for regional studies on spatial variations and comprehensive multispecies research. This study surveyed and monitored wildlife passages across eight nature reserves in Xinjiang, evaluating their construction status, utilization patterns, and key factors influencing utilization rates, as well as providing optimization recommendations. The findings revealed that dedicated wildlife passages were scarce, especially in smaller reserves, which primarily relied on small bridges and culverts originally designed for water flow. Enhancing the construction of passages in these areas is strongly recommended. A total of 32 wildlife species were recorded, comprising 13 bird species and 19 mammal species. Ungulates, including bharal (