Dispersal is a critical process in ecology and evolution, shaping global biodiversity patterns. In stream habitats, which often exist within diverse and fragmented landscapes, dispersal ensures population connectivity and survival. For aquatic insects in particular, landscape features may significantly influence the degree of genetic connectivity among populations. Thus, understanding connectivity drivers in such populations is essential for the conservation and management of streams. We conducted a landscape genetic study using mitochondrial DNA (mtDNA) and genome-wide single nucleotide polymorphism (SNP) markers to assess the functional connectivity of stream insects in a fragmented pasture-dominated landscape. We focused on three species with terrestrial winged adults: the mayfly