Selecting low-nitrogen(N)-tolerant maize hybrids represent an effective approach to enhancing nitrogen use efficiency grain yield. However, the impact of nitrogen fertilization on protein accumulation in low-N-tolerant hybrids remain insufficiently explored. In this paper, a two-year field orientation trial was conducted at four nitrogen fertilizer rate with the different low-N-tolerant maize hybrids. The effect of nitrogen fertilization on the accumulation of protein and its fractions different kernels positions of different low-N-tolerant maize hybrids was studied. The results showed that the protein yield of ZH311 maize kernels was significantly higher than that of XY508, especially under low-N conditions (0N and 150N), and was 25.7%-36.2% higher than that of XY508. There was a significant correlation between protein yield and the accumulation of crude protein and protein fractions. Compared with XY508, the crude protein of ZH311 entered the rapid growth stage later and lasted for a relatively shorter period, but it was 50.8%-53.0% higher due to its higher accumulation rates (v