Xác định tỷ lệ nhiễm Streptococcus suis type 2 ở lợn giết mổ trên địa bàn thành phố Huế và tạo dòng, biểu hiện gene mã hóa 6-phosphogluconate - dehydrogenase protein trong E.Coli BL21=Determination of prevalence of Streptococcus suis type 2 in slaughtered pigs in Hue city and cloning, expression of gene encoding 6-phosphogluconate-dehydrogenase protein in E. coli BL 21

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Tác giả: Thanh Long Đặng, Thị Bích Lân Đinh, Thị Thùy Nhung Hoàng, Văn Chương Huỳnh, Công Thịnh Lê, Quốc Việt Lê, Xuân Hòa Nguyễn, Thăng Long Phùng

Ngôn ngữ: vie

Ký hiệu phân loại:

Thông tin xuất bản: Tạp chí Khoa học kỹ thuật Thú y, 2017

Mô tả vật lý:

Bộ sưu tập: Báo, Tạp chí

ID: 68335

Thêm vào giỏ

In this study, 100 pig nasal swab samples were examined by PCR technique for detection of Streptococcus suis type 2. The studied result indicated that the prevalence of S. suis type 2 in the slaughtered pigs in Hue city was 9%. From the positive samples with S. suis type 2 through PCR technique, the bacterial strain was isolated, the gene encoding 6-phosphogluconate-dehydrogenase (6PGD) protein of this bacteria was cloned and expressed in E. coli BL21 (DE3). The length of gene encoding 6PGD protein of S. suis type 2 was 1428 bp, this gene encoding a polypeptide chain contained 475 amino acid. Similarity level of the cloned 6PGD gene in comparison with 6PGD gene of Genebank (code gb|CP000407.1) was 99%. The result of SDS electrophoresis showed that expression of the 6PGD gene in E. coli BL21 (DE3) produced a fusion protein having molecule weigh of ~76 kDa.Trong nghiên cứu này, chúng tôi đã sử dụng kỹ thuật PCR để phát hiện Streptococcus suis (S. suis) type 2 trong 100 mẫu dịch mũi của lợn. Kết quả nghiên cứu cho thấy tỷ lệ nhiễm S. suis type 2 ở ở lợn giết mổ trên địa bàn thành phố Huế là 9%. Từ những mẫu phân tích bằng kỹ thuật PCR cho kết quả dương tính với S. suis type 2, chúng tôi đã phân lập vi khuẩn thuần, tạo dòng và biểu hiện gene mã hóa kháng nguyên bề mặt 6-phosphogluconate-dehydrogenase (6PGD) của vi khuẩn S. suis type 2 trong E. coli BL21 (DE3). Gene mã hóa kháng nguyên 6PGD phân lập được có độ dài 1428 bp, mã hóa chuỗi polypeptide chứa 475 amino acid, tương đồng 99% so với gene 6PGD được công bố trên GeneBank (mã số gb|CP000407.1). Kết quả phân tích điện di SDS cho thấy protein dung hợp 6PGD-GST biểu hiện bởi tế bào E. coli BL21 (DE3) có khối lượng phân tử khoảng 76 kDa.

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