BACKGROUND/AIMS: Chemical burns to the eye cause extensive ocular surface damage, often resulting in permanent visual impairment. The amniotic membrane (AM), with its regenerative properties, has shown great potential in treating such injuries. Recent innovations, including hydrogel-based delivery systems, have been developed to improve drug retention and promote corneal healing. This study aimed to evaluate whether hydrogels loaded with proteins from human AM could enhance corneal wound healing effectively. METHODS: Alkaline burns (8 mm diameter) were induced in the cornea of anesthetized male New Zealand White rabbits ( RESULTS: There was more frequent corneal wound closure in animals treated with the hydrogels (treatments 3 and 4) on day 14 (44.4 and 55.5%, respectively) than in the untreated controls (33.3%). Histologically, abnormal re-epithelialization and altered epithelial junctions were observed, with no significant differences in epithelial thickness. Endothelial damage correlated with significant thinning ( CONCLUSION: Applying new biocompatible hydrogels to the ocular surface that release AM proteins may help close corneal wounds caused by caustic burns. The aggressive nature of burns hinders the detection of differences in the wound area between treatments 28 days after injury. However, improving the adhesion of a solid hydrogel to the mucosa of the ocular surface could enhance these outcomes by keeping the implant in contact with the wound for a longer period.