Identification and phylogenetic analysis of marine sponges in the Jordanian Gulf of Aqaba using DNA barcoding.

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Tác giả: Abdalmajeed Alajlouni, Neda'a Al-Ajlouni, Farah Alsoqi, Zeinab Arabeyyat, Maysoon Kteifan, Marwan Mahmoud, Sura Shartooh, Mais Sweiss

Ngôn ngữ: eng

Ký hiệu phân loại: 133.594 Types or schools of astrology originating in or associated with a

Thông tin xuất bản: England : Heliyon , 2025

Mô tả vật lý:

Bộ sưu tập: NCBI

ID: 684024

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Sponges (Porifera) are the largest biomass component of coral reefs benthic fauna among marine organisms and are very morphologically diverse. In the present work, we aimed to identify marine sponges in the Jordanian Gulf of Aqaba using the partial 18S rRNA and the 28S rRNA genes as DNA barcoding markers. A total of nine morphologically different marine sponge samples from 6.6m to approximately 22m depth were collected. Sponge fragments were frozen at -80 °C prior to DNA extraction. The sponge's DNA was extracted using a commercial kit and subjected directly to PCR amplification for the 18S rRNA and 28S rRNA genes. The DNA sequences were analyzed using the Basic Local Alignment Search Tool (BLAST) to determine the sponge's identity, and phylogenetic trees were constructed to clarify the relationship among the samples. The results obtained revealed the presence of the following genera:

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